4KF4
Crystal Structure of sfCherry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.105, 96.294, 105.957 |
| Unit cell angles | 90.00, 104.56, 90.00 |
Refinement procedure
| Resolution | 45.260 - 1.994 |
| R-factor | 0.2232 |
| Rwork | 0.222 |
| R-free | 0.26480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: dev_1338)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.030 |
| High resolution limit [Å] | 1.994 | 2.000 |
| Rmerge | 0.095 | 0.546 |
| Number of reflections | 97672 | |
| <I/σ(I)> | 10.45 | 1.65 |
| Completeness [%] | 97.0 | 98.1 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.1 M SPG buffer pH 5.0, 25 % PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
