4KEJ

Crystal structure of mouse Ryanodine Receptor 2 (1-217) disease mutant R169Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Detector technology	CCD
Collection date	2009-12-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97949
Spacegroup name	I 41
Unit cell lengths	112.859, 112.859, 38.094
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.902 - 2.553
R-factor	0.2385
Rwork	0.236
R-free	0.29670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3im7
RMSD bond length	0.003
RMSD bond angle	0.696
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.550	5.490	2.550
Number of reflections	7914
Completeness [%]	98.8	99.9	90
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	0.2 M sodium malonate, 5-15% saturated ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K