4KEA
Crystal structure of D196N mutant of Monoglyceride lipase from Bacillus sp. H257 in space group P212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.165, 183.131, 244.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.882 - 1.700 |
| R-factor | 0.1919 |
| Rwork | 0.191 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.954 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 19.882 | 1.790 |
| High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
| Rmerge | 0.035 | 0.406 | |
| Number of reflections | 195210 | ||
| <I/σ(I)> | 22.6 | 15.4 | 1.9 |
| Completeness [%] | 99.9 | 98.1 | 100 |
| Redundancy | 8.2 | 12.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.9 | 293 | 0.1 M HEPES pH 6.9, 54% v/v MPD, VAPOR DIFFUSION, temperature 293K |
