4KDT
Structure of an early native-like intermediate of beta2-microglobulin amyloidosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 96.790, 96.790, 167.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.430 - 2.600 |
| R-factor | 0.22847 |
| Rwork | 0.226 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x89 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 25281 | |
| <I/σ(I)> | 1.01 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 14.3 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES, 1.6 M MgSO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
