4KC5
Crystal structure of the C-terminal part of RhiE from Burkholderia rhizoxinica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 125.510, 144.130, 131.300 |
| Unit cell angles | 90.00, 96.65, 90.00 |
Refinement procedure
| Resolution | 48.350 - 2.140 |
| R-factor | 0.18213 |
| Rwork | 0.181 |
| R-free | 0.22716 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 252811 | |
| <I/σ(I)> | 2.02 | |
| Completeness [%] | 99.2 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% (w/v) PEG 2000 MME, 100 mM Tris/HCl pH 8.5, 100 mM Trismethylamine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
