4KBM
Structure of the Mtb CarD/RNAP Beta subunit B1-B2 domains complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-09 |
Detector | MAR 300 |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.152, 128.889, 225.526 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.588 - 2.115 |
R-factor | 0.2095 |
Rwork | 0.208 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kbj |
RMSD bond length | 0.005 |
RMSD bond angle | 0.872 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (Phaser) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.590 | 2.210 |
High resolution limit [Å] | 2.110 | 2.110 |
Number of reflections | 40844 | |
<I/σ(I)> | 2.03 | |
Completeness [%] | 98.1 | 92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 14% PEG 3350, 2% tacsimate pH 5.0, 0.1 M sodium citrate tribasic dihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |