4KB5
Crystal structure of MycP1 from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.114, 85.114, 193.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.18955 |
| Rwork | 0.188 |
| R-free | 0.21410 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.890 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.071 | 0.507 |
| Number of reflections | 39312 | |
| <I/σ(I)> | 25.9 | 4.1 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 283 | 1.6 M Magnesium sulfate, 0.1 M MES pH 5.5 with a protein concentration of 20 mg ml-1, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
