4KB2
Crystal structure of ribosome recycling factor mutant R109A from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-01-23 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.490, 33.740, 61.150 |
Unit cell angles | 90.00, 113.22, 90.00 |
Refinement procedure
Resolution | 51.970 - 2.300 |
R-factor | 0.2279 |
Rwork | 0.226 |
R-free | 0.26686 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wqg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.212 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.200 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.165 | 0.165 |
Number of reflections | 9680 | |
<I/σ(I)> | 6.9 | 6.9 |
Completeness [%] | 98.6 | |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Under oil using microbatch plates | 7.8 | 298 | 6% Polyethylene glycol 4000, 20mM Tris-HCl (pH 7.8), 0.6mM Cadmium acetate, 5% Polyethylene glycol 400, Under oil using microbatch plates, temperature 298K |