4KAR
Crystal structure of FDTS (TM0449) mutant (H53D) with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.990, 116.640, 141.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.150 - 2.030 |
| R-factor | 0.19801 |
| Rwork | 0.195 |
| R-free | 0.24876 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o26 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.571 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.200 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.071 | 0.881 |
| Number of reflections | 58450 | |
| <I/σ(I)> | 20.1 | 1.87 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 6.1 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50 - 60% PEG200, 0.1Tris.HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
