4K8Z
KOD Polymerase in binary complex with dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.870, 111.780, 67.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.050 - 2.290 |
| R-factor | 0.1827 |
| Rwork | 0.181 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wns |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.720 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1243)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.324 | 2.350 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.135 | 1.888 |
| Number of reflections | 49755 | |
| <I/σ(I)> | 16.65 | 1.81 |
| Completeness [%] | 99.7 | 96.7 |
| Redundancy | 16.15 | 13.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 50mM Na-Cacodylate, 20mM MgCl2, 1mM Cobalt(III)-hexamminechloride, 1mM Spermine, 20%v/v 2-propanol, , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
