4K8U

Crystal structure of TRAF4 TRAF domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	110
Detector technology	CCD
Collection date	2013-04-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	58.949, 87.989, 117.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.589 - 2.302
R-factor	0.2397
Rwork	0.237
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.451
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	27677
Completeness [%]		95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	13% PEG 3350, 0.14M Magnessium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K