4K84
Crystal structure of human ceramide-1-phosphate transfer protein (CPTP) in complex with 16:0 ceramide-1-phosphate (16:0-C1P)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.2398 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.604, 50.291, 62.870 |
Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
Resolution | 40.278 - 1.897 |
R-factor | 0.1647 |
Rwork | 0.162 |
R-free | 0.21330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Human ceramide-1-phosphate transfer protein in complex with C2 Ceramide-1-phosphate (2:0 C1P) |
RMSD bond length | 0.007 |
RMSD bond angle | 0.936 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.079 | 0.047 | 0.269 |
Number of reflections | 30620 | ||
<I/σ(I)> | 10 | ||
Completeness [%] | 99.7 | 99.7 | 96.8 |
Redundancy | 6.8 | 7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1 M MMT buffer pH 5.0, 25% PEG1500, VAPOR DIFFUSION, SITTING DROP, temperature 293K |