4K82
Crystal structure of lv-ranaspumin (Lv-RSN-1) from the foam nest of Leptodactylus vastus, monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.874, 25.848, 66.076 |
| Unit cell angles | 90.00, 103.98, 90.00 |
Refinement procedure
| Resolution | 25.629 - 1.600 |
| R-factor | 0.1896 |
| Rwork | 0.187 |
| R-free | 0.23730 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.033 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.119 | 45.259 | 1.690 |
| High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
| Rmerge | 0.060 | 0.781 | |
| Total number of observations | 3721 | 18307 | |
| Number of reflections | 22985 | ||
| <I/σ(I)> | 9.8 | 9.1 | 1 |
| Completeness [%] | 99.9 | 96.3 | 100 |
| Redundancy | 5.5 | 4.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapour diffusion, sitting drop | 7.3 | 293 | 0.2 M Potassium formate, 20% (w/v) PEG 3350, pH 7.3, vapour diffusion, sitting drop, temperature 293K |
