4K6N
Crystal structure of yeast 4-amino-4-deoxychorismate lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97838 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.966, 113.893, 86.004 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.790 - 1.900 |
| R-factor | 0.1744 |
| Rwork | 0.173 |
| R-free | 0.20180 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.259 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.422 |
| Number of reflections | 35258 | |
| <I/σ(I)> | 20 | 4.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289.15 | 20%(w/v) polyethylene glycol monomethyl ether 5000, 0.1M Bis-Tris, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 289.15K |
