4K68

Structure of a novel GH10 endoxylanase retrieved from sugarcane soil metagenome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.459
Spacegroup name	P 1 21 1
Unit cell lengths	41.877, 116.442, 82.811
Unit cell angles	90.00, 99.85, 90.00

Refinement procedure

Resolution	41.200 - 2.740
R-factor	0.2062
Rwork	0.204
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.588
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.200	2.850
High resolution limit [Å]	2.740	2.740
Rmerge	0.143	0.468
Number of reflections	20118
Completeness [%]	97.6	87.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	292	PEG8000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K