4K5U
Recognition of the BG-H Antigen by a Lamprey Variable Lymphocyte Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.710, 62.784, 63.366 |
| Unit cell angles | 72.03, 71.39, 84.35 |
Refinement procedure
| Resolution | 59.720 - 1.698 |
| R-factor | 0.20566 |
| Rwork | 0.206 |
| R-free | 0.24661 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e6j |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.220 |
| Data reduction software | CrystalClear ((Rigaku)) |
| Data scaling software | CrystalClear ((Rigaku)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.720 | 1.760 |
| High resolution limit [Å] | 1.698 | 1.698 |
| Number of reflections | 82662 | |
| Completeness [%] | 93.3 | 87.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 28.5% (w/v) PEG 3350, 0.6 M NaCl, and 0.1M Tris-HCl (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
