4K4Z
Coxsackievirus B3 polymerase elongation complex (r2_Mg_form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | NOIR-1 |
Spacegroup name | P 1 |
Unit cell lengths | 60.641, 60.633, 194.680 |
Unit cell angles | 89.98, 89.92, 78.68 |
Refinement procedure
Resolution | 45.580 - 2.170 |
R-factor | 0.206 |
Rwork | 0.204 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.102 |
Data reduction software | d*TREK (9.9.9.4D) |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 2.250 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.055 | 0.474 |
Number of reflections | 140661 | |
<I/σ(I)> | 7.7 | 1.5 |
Completeness [%] | 97.8 | 97.1 |
Redundancy | 1.95 | 1.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | sitting drop, temperature 289K, VAPOR DIFFUSION, SITTING DROP |