4K3X

Crystal structure of a subtype H18 hemagglutinin homologue from A/flat-faced bat/Peru/033/2010 (H18N11)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03318
Spacegroup name	I 4 2 2
Unit cell lengths	238.884, 238.884, 160.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.940 - 2.149
R-factor	0.1851
Rwork	0.183
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rd8
RMSD bond length	0.008
RMSD bond angle	1.074
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.149	2.149
Number of reflections	121108
Completeness [%]	97.1	79.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.9	295	0.1 M MES, pH 6.9, 5% PEG1000, 27.5% PEG600, VAPOR DIFFUSION, temperature 295K