4K3S

E. coli sliding clamp in P1 crystal space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-18
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95
Spacegroup name	P 1
Unit cell lengths	40.980, 64.970, 71.930
Unit cell angles	73.81, 82.46, 84.04

Refinement procedure

Resolution	28.240 - 1.750
R-factor	0.1966
Rwork	0.195
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mmi
RMSD bond length	0.007
RMSD bond angle	1.247
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.680	28.245	1.840
High resolution limit [Å]	1.750	5.530	1.750
Rmerge		0.042	0.456
Rmeas		0.049	0.539
Rpim		0.026	0.285
Total number of observations		7644	34287
Number of reflections	67726
<I/σ(I)>	10.7	21.2	2.4
Completeness [%]	95.3	96	93.8
Redundancy	3.6	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	285	100mM MES, 100-150mM CaCl2, 25-30%(v/v) PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K