4K39
Native anSMEcpe with bound AdoMet and Cp18Cys peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.031, 91.915, 91.004 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 41.022 - 1.783 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.21490 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.346 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.780 |
| Number of reflections | 69024 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 29% PEG 4000, 150 mM ammonium acetate, 100 mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 298K |
