4K38
Native anSMEcpe with bound AdoMet and Kp18Cys peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.383, 92.068, 91.102 |
Unit cell angles | 90.00, 91.21, 90.00 |
Refinement procedure
Resolution | 41.085 - 1.831 |
R-factor | 0.1732 |
Rwork | 0.172 |
R-free | 0.20540 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.456 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.830 |
Number of reflections | 64439 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 29% PEG 4000, 150 mM ammonium acetate, 100 mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 298K |