4K2J
Decameric ring structure of KSHV (HHV-8) latency-associated nuclear antigen (LANA) DNA binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.439, 175.176, 97.065 |
| Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
| Resolution | 39.890 - 2.050 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.391 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 103035 | |
| <I/σ(I)> | 13.1 | 2.4 |
| Completeness [%] | 97.2 | 97.7 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1M ammonium formate, 100mM HEPES pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
