4K1T
Gly-Ser-SplB protease from Staphylococcus aureus at 1.60 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.740, 77.760, 95.790 |
| Unit cell angles | 90.00, 131.81, 90.00 |
Refinement procedure
| Resolution | 18.910 - 1.600 |
| R-factor | 0.18599 |
| Rwork | 0.184 |
| R-free | 0.22348 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w7s |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.312 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.910 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 81007 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.01M Zinc sulfate heptahydrate, 0.1M MES monohydrate pH 6.5, 25% v/v Polyethylene glycol monomethyl ether 550, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
