4K14
Crystal Structure of Staphylococcal nuclease mutant V66I/V99L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 298 |
Detector technology | DIFFRACTOMETER |
Collection date | 2011-05-02 |
Detector | RIGAKU AFC9 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 47.450, 47.450, 63.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.140 - 1.601 |
R-factor | 0.20971 |
Rwork | 0.207 |
R-free | 0.25152 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.539 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 21.220 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.033 |
Number of reflections | 15733 |
<I/σ(I)> | 12.4 |
Completeness [%] | 84.0 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 50mM phosphate buffer,pH 7.5 45% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 277K |