4JZ4
Crystal structure of chicken c-Src-SH3 domain: monomeric form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.670, 45.358, 58.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.735 - 1.560 |
| R-factor | 0.1526 |
| Rwork | 0.151 |
| R-free | 0.17570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fj5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.202 |
| Data scaling software | SCALA (0.1.30) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.670 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.044 | 0.340 |
| Number of reflections | 16919 | |
| <I/σ(I)> | 16 | 2 |
| Completeness [%] | 99.6 | 96.7 |
| Redundancy | 3.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.8M Ammonium sulphate, 10% Glicerol, 5mM NiCl, 0.1 M Hepes , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
