4JZ0

X-ray structure of human glutamate carboxypeptidase II (GCPII) in complex with CTT1055

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.91953
Spacegroup name	I 2 2 2
Unit cell lengths	101.819, 130.399, 159.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.590 - 1.830
R-factor	0.15261
Rwork	0.152
R-free	0.17097
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.019
RMSD bond angle	1.639
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.830
Number of reflections	85814
Completeness [%]	91.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	33% (v/v) pentaerythritol propoxylate PO/OH 5/4, 0.5% (w/v) PEG 3350, 100mM Tris-HCl , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K