4JYD
X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.598, 78.699, 85.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.600 - 1.710 |
R-factor | 0.19239 |
Rwork | 0.189 |
R-free | 0.25056 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.843 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 1.800 |
High resolution limit [Å] | 1.710 | 1.710 |
Number of reflections | 68817 | |
Completeness [%] | 96.0 | 85.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM Tris; 200 mM Li2SO4; 30% PEG4000, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |