4JXE
Crystal structure of Schizosaccharomyces pombe sst2 catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-10-07 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.139, 69.436, 62.018 |
Unit cell angles | 90.00, 104.84, 90.00 |
Refinement procedure
Resolution | 29.640 - 1.451 |
R-factor | 0.1642 |
Rwork | 0.163 |
R-free | 0.19240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2znr |
RMSD bond length | 0.005 |
RMSD bond angle | 1.052 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP ((ccp4)) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.640 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.060 | |
Number of reflections | 80642 | |
<I/σ(I)> | 19.28 | |
Completeness [%] | 99.7 | 99.2 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M Ammonium phosphate dibasic, 20 % w/v polyethylene glycol 3,350 , 30% w/v 1,6- hexanediol , VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8 |