4JX2

Crystal structure of a putative secreted protein (lpg1979) from Legionella pneumophila subsp. pneumophila str. Philadelphia 1 at 2.65 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-01-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97932,0.91837,0.97858
Spacegroup name	P 21 21 2
Unit cell lengths	121.943, 145.086, 63.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.970 - 2.650
R-factor	0.1642
Rwork	0.162
R-free	0.20140
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.970		2.740
High resolution limit [Å]	2.650	5.670	2.650
Rmerge	0.087	0.039	0.570
Number of reflections	33147	3554	3064
<I/σ(I)>	11.63	25.3	2.3
Completeness [%]	98.1	96.3	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2M lithium sulfate, 40.0% polyethylene glycol 400, 0.2M lithium sulfate, 40.0% polyethylene glycol 400, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K