4JWO
The crystal structure of a possible phosphate binding protein from Planctomyces limnophilus DSM 3776
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 61 |
| Unit cell lengths | 87.158, 87.158, 75.536 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.529 - 1.601 |
| R-factor | 0.1727 |
| Rwork | 0.172 |
| R-free | 0.19460 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.633 |
| Number of reflections | 42889 | |
| <I/σ(I)> | 50.8 | 3.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.3 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 2.0M Ammonium Sulfate, 0.1M Sodium Acetate:HCl, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
