4JWH
Crystal structure of spTrm10(Full length)-SAH complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.825, 45.653, 66.412 |
| Unit cell angles | 90.00, 100.18, 90.00 |
Refinement procedure
| Resolution | 32.680 - 2.040 |
| R-factor | 0.20102 |
| Rwork | 0.198 |
| R-free | 0.25667 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.367 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 4.390 | 2.040 |
| Rmerge | 0.066 | 0.032 | 0.435 |
| Number of reflections | 21488 | ||
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 97.3 | 97.5 | 95.1 |
| Redundancy | 3.6 | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 287 | 15% PEG 8000, 0.1M Na acetate, pH 5.0, vapor diffusion, hanging drop, temperature 287K |
