4JW0
Structure of Gloeobacter violaceus CcmL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.377, 81.619, 89.349 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.297 - 1.700 |
R-factor | 0.1624 |
Rwork | 0.161 |
R-free | 0.19140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.944 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.300 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.014 | |
Number of reflections | 58606 | |
<I/σ(I)> | 0.9 | |
Completeness [%] | 97.4 | 82.4 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.2 M Lithium sulfate, 0.1 M Bis-Tris, 10% (w/v) PEG 3350, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |