4JUT
Crystal structure of a mutant fragment of Human HSPB6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.99 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.440, 86.010, 87.070 |
Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
Resolution | 43.005 - 2.196 |
R-factor | 0.1991 |
Rwork | 0.196 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.164 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.010 |
High resolution limit [Å] | 2.196 |
Number of reflections | 49462 |
Completeness [%] | 99.4 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop vapor diffusion | 7.5 | 277 | 0.1M HEPES (pH 7.5), 0.2M litium nitrate, 20% PEG 3350, hanging drop vapor diffusion, temperature 277K |