4JRF

Crystal structure of a putative cell adhesion protein (BACOVA_01548) from Bacteroides ovatus ATCC 8483 at 1.98 A resolution (PSI Community Target, Nakayama)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979029,0.979338,0.953725
Spacegroup name	I 4
Unit cell lengths	134.022, 134.022, 57.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.384 - 1.980
R-factor	0.1567
Rwork	0.155
R-free	0.17810
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.384		2.050
High resolution limit [Å]	1.980	4.250	1.980
Rmerge	0.099	0.010	0.010
Number of reflections	35859	3724	3507
<I/σ(I)>	13.29	36.4	1.9
Completeness [%]	100.0	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2M lithium sulfate, 30.0% polyethylene glycol 4000, 0.1M tris hydrochloride pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K