4JRC
Distal Stem I region from G. kaustophilus glyQS T box RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9767 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.461, 41.511, 90.417 |
| Unit cell angles | 90.00, 106.76, 90.00 |
Refinement procedure
| Resolution | 20.140 - 2.673 |
| R-factor | 0.2051 |
| Rwork | 0.201 |
| R-free | 0.24270 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.409 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 7.170 | 2.650 |
| Rmerge | 0.068 | 0.026 | 0.484 |
| Number of reflections | 11578 | ||
| <I/σ(I)> | 10.9 | 47.56 | 1.808 |
| Completeness [%] | 97.2 | 96.3 | 82.7 |
| Redundancy | 3.6 | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 80mM NaCl, 12mM KCl, 20mM MgCl2, 40mM sodium cacodylate, 12mM spermine, 28% 2-methyl-1,3 propanediol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
