4JR7

Crystal structure of scCK2 alpha in complex with GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	57.745, 69.512, 94.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.490 - 1.480
R-factor	0.18667
Rwork	0.186
R-free	0.20421
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.320
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.480
Number of reflections	63216
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	287	2mM GMPPNP, 200mM magnesium chloride, 25% w/v polyethylene glycol 3350, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K