4JPR
Structure of the ASLV fusion subunit core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-16 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54 |
Spacegroup name | H 3 |
Unit cell lengths | 42.544, 42.544, 119.982 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.220 - 2.001 |
R-factor | 0.2103 |
Rwork | 0.207 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mof |
RMSD bond length | 0.014 |
RMSD bond angle | 1.363 |
Data reduction software | d*TREK (9.9.9.1D) |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.220 | 35.220 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.026 | 0.019 | 0.308 |
Total number of observations | 3401 | 1130 | |
Number of reflections | 5371 | ||
<I/σ(I)> | 28.6 | 83.1 | 2.7 |
Completeness [%] | 97.9 | 100 | 81.8 |
Redundancy | 5.28 | 5.83 | 2.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% (w/v) MPD, 5% (w/v) PEG 8000, 0.1 M sodium cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |