4JPP

Bacteriophage phiX174 H protein residues 143-282

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00718
Spacegroup name	P 42 21 2
Unit cell lengths	68.699, 68.699, 372.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.2477
Rwork	0.245
R-free	0.29010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.322
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.490
High resolution limit [Å]	2.400	5.160	2.400
Rmerge		0.049	0.327
Number of reflections	35261
<I/σ(I)>	4.3
Completeness [%]	96.5	91.7	99.5
Redundancy	6.7	6.4	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10-15% w/v PEG8000, 0.1 M Tris-HCl, pH 7.5, 20% w/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K