4JOM

Structure of E. coli Pol III 3mPHP mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	82.429, 98.944, 139.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.945 - 2.900
R-factor	0.1966
Rwork	0.194
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hnh
RMSD bond length	0.003
RMSD bond angle	0.733
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.945	3.070
High resolution limit [Å]	2.900	2.900
Number of reflections	26274
Completeness [%]	99.6	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	10-15 mg/mL protein, 15-20% PEG3350, 0.2-0.4 M sodium phosphate monobasic, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, temperature 293K