4JO8

Crystal structure of the activating Ly49H receptor in complex with m157 (G1F strain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9436
Spacegroup name	P 31 1 2
Unit cell lengths	87.870, 87.870, 133.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.190 - 3.200
R-factor	0.2272
Rwork	0.226
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nyk
RMSD bond length	0.009
RMSD bond angle	1.070
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	76.100
High resolution limit [Å]	3.200
Number of reflections	9772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M HEPES 0.5% PEG4000, 10% 2-propanol, 50mM magnesium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K