4JO7

Crystal structure of the human Nup49CCS2+3* Nup57CCS3* complex with 2:2 stoichiometry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-04
Detector	PSI PILATUS 6M
Wavelength(s)	0.9805
Spacegroup name	P 1
Unit cell lengths	47.689, 47.658, 71.324
Unit cell angles	88.87, 83.37, 81.42

Refinement procedure

Resolution	47.125 - 1.752
R-factor	0.1873
Rwork	0.186
R-free	0.21440
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.804
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8_1069)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.750
Number of reflections	117121

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.1 M MES, pH 6.5, 6.7% PEG20000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K