4JNX
Crystal structure of RNA silencing suppressor p19 complexed with double-helical RNA 20mer pG(CUG)6C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98035 |
| Spacegroup name | H 3 |
| Unit cell lengths | 89.917, 89.917, 148.353 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.300 - 1.950 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r9f |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.190 | 1.950 |
| Rmerge | 0.072 | 0.035 | 0.726 |
| Number of reflections | 32285 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 98.9 | 98.8 | 98.2 |
| Redundancy | 2.9 | 2.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 291 | 1.6 M ammonium sulfate, pH 8, potassium chloride, MES/NaOH, trace magnesium, HANGING DROP, temperature 291K |
