4JMR

A unique spumavirus gag N-terminal domain with functional properties of orthoretroviral Matrix and Capsid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-18
Detector	PSI PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	59.411, 122.365, 61.766
Unit cell angles	90.00, 97.52, 90.00

Refinement procedure

Resolution	45.530 - 2.900
R-factor	0.2285
Rwork	0.226
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.839
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.530	45.500	3.000
High resolution limit [Å]	2.900	10.000	2.900
Rmerge	0.064	0.031	0.322
Number of reflections	19334	474	1891
<I/σ(I)>	14.55	29.37	4.28
Completeness [%]	99.3	96.7	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	289	500uM 1:1 complex mixed with an equal volume 10% PEG 4000, 20% glycerol, 30mM MgCl2, 30mM CaCl2, 100mM Tris-Bicine pH 8.5, VAPOR DIFFUSION, temperature 289K