4JM0
Structure of Human Cytomegalovirus Immune Modulator UL141
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-03 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 96.060, 96.060, 136.071 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.980 - 3.250 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.352 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.980 | 3.330 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 10665 | |
| Completeness [%] | 75.0 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 295.15 | 0.2 M calcium acetate, 0.1 M imidazole pH 8, 10% (w/v) polyethylene glycol 8000, VAPOR DIFFUSION, SITTING DROP, temperature 295.15K |
