4JLQ

Crystal structure of human Karyopherin-beta2 bound to the PY-NLS of Saccharomyces cerevisiae NAB2

Replaces:  4H1K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-07
Detector	ADSC QUANTUM 315
Wavelength(s)	1.07500
Spacegroup name	P 21 21 2
Unit cell lengths	132.234, 172.383, 68.443
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.600 - 3.050
R-factor	0.1867
Rwork	0.184
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2QMR and 4FDD
RMSD bond length	0.011
RMSD bond angle	1.538
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0025)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.050	3.050
Number of reflections	30914
<I/σ(I)>	16.6	1.18
Completeness [%]	100.0	100
Redundancy	8.8	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	298	CRYSTALLIZATION CONDITIONS: PROTEIN (20 MM HEPES, PH 7.3, 110 MM POTASSIUM ACETATE, 2MM MAGNESIUM ACETATE, 20% GLYCEROL, 2MM DTT); RESERVOIR (MCSG3 #95: 0.7 M SODIUM CITRATE TRIBASIC, 100MM BIS-TRISPROPANTE pH 7.0); CRYOPROTECTION (20% GLYCEROL), temperature 298K, VAPOR DIFFUSION, SITTING DROP