4JL9
Crystal structure of mouse TBK1 bound to BX795
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2012-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 140.782, 140.782, 86.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.082 - 3.100 |
| R-factor | 0.2309 |
| Rwork | 0.227 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.683 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.099 | 3.099 |
| Rmerge | 0.064 | 1.000 |
| Number of reflections | 18262 | |
| <I/σ(I)> | 40 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M sodium citrate, 10-14% isopropanol, 0.1 M HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
