4JKX
Crystal structure Mistletoe Lectin I from Viscum album in complex with kinetin at 2.35 A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.826 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 107.010, 107.010, 312.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 79.700 - 2.350 |
R-factor | 0.22179 |
Rwork | 0.220 |
R-free | 0.24822 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eb2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.320 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 79.700 | 2.400 | |
High resolution limit [Å] | 2.350 | 10.000 | 2.350 |
Rmerge | 0.082 | 0.013 | 0.013 |
<I/σ(I)> | 14.09 | 53.64 | 1.11 |
Completeness [%] | 99.9 | 98 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 2.5 | 293 | 1.0M ammonium sulphate, 0.2M glycine/HCl, pH 2.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |