4JKR

Crystal Structure of E. coli RNA Polymerase in complex with ppGpp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	P 21 21 21
Unit cell lengths	187.575, 206.164, 311.062
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.970 - 4.200
R-factor	0.25027
Rwork	0.247
R-free	0.31802
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.935
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.970
High resolution limit [Å]	4.200
Number of reflections	88171
Completeness [%]	99.7
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	20% PEG400, 0.18M strontium chloride, 0.1M HEPES , pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K