4JKL
Crystal Structure of Streptococcus agalactiae beta-glucuronidase in space group P21212
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-11-29 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.001, 198.759, 75.573 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.306 - 2.288 |
R-factor | 0.1769 |
Rwork | 0.174 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.729 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.380 |
High resolution limit [Å] | 2.288 | 4.960 | 2.300 |
Rmerge | 0.158 | 0.076 | 0.476 |
Number of reflections | 57552 | ||
<I/σ(I)> | 4.7 | ||
Completeness [%] | 98.3 | 99.6 | 90.9 |
Redundancy | 12.4 | 14.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 20-28% PEG 3350, 0.1-0.3 M KSCN, 0.02% sodium azide, vapor diffusion, hanging drop, temperature 289.15K |