4JKK
Crystal Structure of Streptococcus agalactiae beta-glucuronidase in space group I222
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-10 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.814, 83.829, 199.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.670 - 2.590 |
| R-factor | 0.15 |
| Rwork | 0.146 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.436 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.670 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Rmerge | 0.128 | 0.363 |
| Number of reflections | 18183 | |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 6.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 289.15 | 20-28% PEG 3350, 0.1-0.3 M KSCN, 0.02% sodium azide, vapor diffusion, hanging drop, temperature 289.15K |
